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KMID : 0369819900200020273
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Jorunal of Korean Pharmaceutical Sciences
1990 Volume.20 No. 2 p.273 ~ p.278
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Fluorometric Determination of D- Penicillamine with 9 - Fluorenylmethyl Pentafluorophenyl Carbonate
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변승연/Byeon SY
최중갑/유경수/Choi JK/Yoo GS
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Abstract
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A sensitive fluorometric method using 9-fluorenylmethyl pentafluorophenyl carbonate (FMPC) as the fluorescent labeling agent was developed to determine D-penicillamine (D-PA). The fluorophore had excitation and emission wavelengths of 260 ㎚ and 313 ㎚, respectively. After derivatization, the fluorescent product was separated, and quantified by spectrofluorometry. The derivative was highly fluorescent and stable. Optimum condition for the reaction was investigated. A linear response was obtained over the range of 4.0 × 10^(-7)-5.0 × 10^(-6) M with the correlation coefficient of 0.999 (n = 6). The procedure described was successfully applied to the determination of the dosage forms of capsule with the recovery of 98.62 ± 0.57% (150 ㎎), 98.36±0.57% (250 ㎎).
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KEYWORD
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